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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Overexpression of the gene continues to be noticed in several tumors, which leads to chromosome instability, and so may contribute to tumorigenesis. This protein was discovered to affiliate with, and become linked to, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in mobile-cycle regulated histone gene expression and plays a crucial function in advertising cell-cycle development while in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinct isoforms, have been explained. Two added splice variants had been noted but detailed nucleotide sequence information and facts will not be but accessible.

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Blocking peptides are peptides that bind specially to the goal antibody and block antibody binding. These peptide commonly consists of the epitope acknowledged because of the antibody.

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This guidebook outlines even further probable optimization of mobile and immunoassay parameters to ensure the best possible outcomes are...

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This guidebook outlines even more doable optimization of mobile and immunoassay parameters to be certain the very best success are...

Antibodies bound to the blocking peptide now not bind for the epitope within the concentrate on protein. By evaluating the staining with the blocked antibody as opposed to the antibody on your own, one can see which staining is particular.

Target Information Cyclin E belongs to the really conserved cyclin spouse and children, whose customers are characterized by a remarkable periodicity in protein abundance through the cell cycle. Cyclins functionality as regulators of CDK kinases. Unique cyclins exhibit distinct expression and degradation styles which lead towards the temporal coordination of each and every mitotic celebration. Cyclin E kinds a complex with and capabilities being a regulatory subunit of CDK2, whose activity is needed for mobile cycle G1/S changeover. This protein accumulates on the G1-S phase boundary which is degraded as cells progress through S period.

The measurement of protein phosphorylation is a great website tool for measuring the modulation of receptor activation by each antibodies...

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